Fast and Efficient Elution of Proteins from Polyacrylamide Gels Using Nanosep® Centrifugal Devices
نویسندگان
چکیده
Polyacrylamide Gel Electrophoresis (PAGE) is both an analytical and preparative tool widely used in protein analysis. Proteins are separated by PAGE in an electric field according to their size, shape and charge both in their native configuration (native PAGE) or in the presence of denaturing agents such as the ionic detergent sodium dodecyl sulfate (SDS-PAGE). A third type of electrophoretic technique, isoelectric focusing (IEF), separates proteins according to differences in their isoelectric points.
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